Resources
Access essential tools and resources to support your research.Shared equipment
The Shared Equipment Cost Center at HRT/3NJB provides a pool of research tools available to the participant labs. In addition to the items below, the cost center also funds -80 freezer repairs (up to $1000 per incident) and the repair of biosafety cabinets (to meet EH&S compliance standards). For specific equipment info, please contact the caretaker identified with each listing. For program inquiries contact Matthew Curry, Research Operations Manager, mcurry@uw.edu or 206.685.8769.
Automated western blotting
ProteinSimple Jess, a fully-automated, capillary-based, multiplex, western blot system. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Centrifuges
Sorvall WX 80 Ultra available in 3NJB COR7. Please contact Olusegun Soge at 206.897.5325 or sogeo@uw.edu.
RC-6+ Single-Phase, High-Speed centrifuge, 3NJB COR7. Please contact Olusegun Soge at 206.897.5325 or sogeo@uw.edu.
Sorvall WX 80 Plus Ultracentrifuge, HRT 510. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Beckman Optima XL Ultracentrifuge, HRT 518. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Digital imaging
Li-Cor Odyssey CLx Imagers (x2) in HRT 510. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Li-Cor Odyssey CLx near-infrared imager in 3NJB. Cell-based assays, protein arrays, in-gel imaging (when appropriate detection dye is used), tissue section imaging. The standard wavelengths detected are 680 and 800. Labs are welcome to come and try to see if it will work for their applications, but it’s recommended that a little research be done first. Contact Jessica Wagoner at 206.897.5219 or jw25@uw.edu.
DNA/RNA isolation
King Fisher Duo, in HRT 510. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Electrophoresis
Agilent Bioanalyzer located in HRT 510, Bench 1. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Gamma/scintillation counter
Perkin Elmer LS6000, HRT 600. Please contact Lorenzo Giacani for details at giacal@uw.edu.
Glass washers
Located in HRT rooms 028, 320-720, and 3NJB 343, Please contact the Facilities Manager, Mike Cheney, cheneym@uw.edu with questions.
Label maker
Brady Label Maker/Printer located in HRT 510 at the Tech Desk across from the RT-PCR machines. Users need to provide their own consumables, namely labels and ink. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
PCRs
ABI Real-Time PCR ViiA 7, 96-well located in the HRT 510. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu
ThermoFisher QuantStudio7 RT-PCR in HRT 510. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
QuantStudio 7 Flex RT-PCR located in 3NJB 324. Contact Olusegun Soge at 206.897.5325 or sogeo@uw.edu.
Plate reader
Victor Nivo multimode plate reader system located in HRT 510. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Spare freezers
There are two spare -80 freezers and one spare -20 freezer located in the HRT 5th floor East corridor. There is also one spare -80 and one spare -20 in the HRT 208 freezer farm.
Another spare -80 freezer is in the 3NJB main N-S hallway.
All spare freezers are for short-term/temporary storage of samples, in case of a planned defrost or unexpected failure. Sign-in sheets on the doors must be filled in with contact info when placing samples, in case we need to contact you. Please contact Matthew Curry at 206.685.8769 or mcurry@uw.edu, with questions.
Spectrophotometer
ThermoFisher NanoDrop located in HRT 510, Bench 1. Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Water filtration stations
Barnstead Nanopure Diamond filtration unit located in 3NJB 343, and another in HRT 620.
Please contact Kristyn Ringgold at 206.897.5034 or kringgo@uw.edu.
Additional UW research resources
A listing of equipment, facilities, and services available across the UW research community.
Shared Research Facilities and Resources